貨號 | 品牌 | CAS | 規格 | 銷(xiāo)售價(jià)(RMB) | 折扣價(jià)(RMB) | 備注 | 數量 |
KTI2034-100T | Abbkine | 無(wú) | 100T | 3698 | 3698 | 無(wú) | |
KTI2034-20T | Abbkine | 無(wú) | 20T | 958 | 958 | 無(wú) |
產(chǎn)品英文名稱(chēng) | IPKine? Anti-GST Magnetic IP Kit |
免疫原 | 合成多肽 |
反應性 | Mammals, Bacteria |
應用 | IP |
實(shí)驗建議 | 谷胱甘肽巰基轉移酶標簽(GST-Tag,GST標簽)小鼠單克隆抗體通過(guò)共價(jià)鍵偶聯(lián)磁珠,可用于免疫沉淀檢測和捕獲含有GST-Tag序列的融合蛋白,優(yōu)化后的Anti-GST Magnetic Beads具有更高效的抗原結合能力。根據目的蛋白的結構、生物學(xué)功能及后續應用的要求等,本試劑盒提供兩種洗脫方法,包括酸洗脫和SDS-PAGE Loading Buffer洗脫方法對目的蛋白進(jìn)行洗脫。特別是酸洗脫后不會(huì )包含抗體的輕鏈和重鏈,可以有效解決免疫沉淀后Western Blotting實(shí)驗中抗體重鏈和輕鏈的干擾問(wèn)題。 |
偶聯(lián)物 | 磁珠 |
試劑盒組分 | Non-Denaturing Lysis Buffer TBS (10×) Anti-GST Magnetic Beads Mouse IgG Magnetic Beads Elution Buffer Neutralization Buffer SDS-PAGE Loading Buffer (5×) |
特點(diǎn)&優(yōu)勢 | ? 高效:特異性強、靶蛋白結合量高,≥0.6 mg GST標簽融合蛋白/mL磁珠; ? 通用:提供IP實(shí)驗所需的所有必要緩沖液; ? 可靠:提供陰性對照,可排除IgG本身和目的蛋白或其它特定生物分子的非特異性結合; ? 靈活:本試劑盒提供兩種洗脫方法(酸洗脫和SDS-PAGE Loading Buffer洗脫方法)。 |
保存建議 | 按各組分標簽提示分開(kāi)存儲,保質(zhì)期12個(gè)月。 |
運輸條件 | 藍冰運輸 |
警告 | 本產(chǎn)品僅供科學(xué)研究使用,不適用于臨床診斷。 |
背景 | The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins. |
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